This is the first time that Owlette becomes the leader of the PJ Masks.
This is also the first time that the Ninjalinos finally stand up to Night Ninja. However, Owlette doesn't prove to be a very good leader, and starts acting similarly to how Night Ninja did. They do eventually go back to Night Ninja, when he apologizes.
A villain (the Ninjalinos) swap teams for the first time in this episode.
This episode mentions Egyptian culture for the first time.
It is revealed that Night Ninja has a soft side. Owlette even mentions it, which ends up offending him.
The museum is revealed to have a model Space Shuttle. It is functional; Night Ninja and his Ninjalinos can be seen riding in it before the engines failed.